LOCUS       40924_255        5021 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Boolean integrase logic vector AND gate BCD-RFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 5021)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5021)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..69
                     /label=P7
                     /note="P7"
                     /regulatory_class="promoter"
     misc_feature    76..128
                     /label=attB TP901-1
                     /note="attB TP901-1"
     terminator      137..208
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      224..251
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    258..307
                     /label=attP TP901-1
                     /note="attP TP901-1"
     misc_feature    314..363
                     /label=attB Bxb1
                     /note="attB Bxb1"
     regulatory      364..476
                     /label=j61048
                     /note="j61048"
                     /regulatory_class="terminator"
     misc_feature    477..529
                     /label=attP Bxb1
                     /note="attP Bxb1"
     misc_RNA        531..581
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     misc_feature    605..689
                     /label=BCD2
                     /note="BCD2"
     RBS             675..683
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             687..1382
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     terminator      complement(1408..1451)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1492..1519
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1526..1546
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1582..1613
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      1738..1832
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2324..2546
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2594..3541
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     CDS             3833..4690
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4704..4763
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      4823..4852
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      complement(4946..4975)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agaaaaaatt tatttgcttt cgcatctttt tgtacctata
       61 atgtgtggaa agcttatgcc aacacaatta acatctcaat caaggtaaat gctttttgct
      121 ttttttgccc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      181 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      241 gcctttctgc gtttataaaa ggagtttttt agttacctta attgaaataa acgaaataaa
      301 aactcgcgct agctcggccg gcttgtcgac gacggcggtc tccgtcgtca ggatcatccg
      361 ggcccggctt atcggtcagt ttcacctgat ttacgtaaaa acccgcttcg gcgggttttt
      421 gcttttggag gggcagaaag atgaatgact gtccacgacg ctatacccaa aagaaagggt
      481 ttgtaccgta caccactgag accgcggtgg ttgaccagac aaaccacgaa gctgtcaccg
      541 gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca gcctctacaa ataattttgt
      601 ttaagggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt cgtactgaaa
      661 catcttaatc atgctaagga ggttttctaa tgtcagaatt aattaaagaa aatatgcaca
      721 tgaaattata tatggaaggt actgtcaaca atcatcattt caaatgcaca tccgaaggtg
      781 aaggtaaacc atatgaaggc acacaaacaa tgcgcatcaa agcagttgaa ggtggacccc
      841 tgccctttgc gtttgacatt ctcgcaacga gctttatgta cgggtctaaa acttttatca
      901 atcacaccca aggcattcct gactttttta aacagtcctt tcctgaaggc tttacctggg
      961 aacgtgtaac aacttatgaa gatggcggtg tacttacagc aactcaagat acgagtttac
     1021 aagatggctg tctgatttac aatgttaaaa tccgtggcgt aaatttcccg agtaacggac
     1081 ccgtaatgca aaaaaaaact cttggttggg aagcatcaac agaaacctta tatcctgcgg
     1141 acggtggctt agaaggacgc gcagacatgg cactgaaatt agttggaggc ggtcatttaa
     1201 tctgcaacct gaaaacaacc tatcgttcca aaaaacccgc taaaaacctt aaaatgcctg
     1261 gagtatacta tgttgatcgt cgcttagaac gtattaaaga agctgataaa gaaacctacg
     1321 ttgaacaaca tgaagtagcc gtagcccgtt attgtgacct tccgtcgaaa ttaggacatc
     1381 gttgataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1441 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1501 tcgggtgggc ctttctgcgt ttatatacta gtagcggccg ctgcaggagt cactaagggt
     1561 tagttagtta gattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt
     1621 cccttggggt tatcattggg gctcactcaa aggcggtaat cagataaaaa aaatccttag
     1681 ctttcgctaa ggatgatttc tgctagagct gtcagaccaa gtttacgagc tcgcttggac
     1741 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
     1801 gttgccgccg ggcgtttttt attggtgaga atccaagcac tagggacagt aagacgggta
     1861 agcctgttga tgataccgct gccttactgg gtgcattagc cagtctgaat gacctgtcac
     1921 gggataatcc gaagtggtca gactggaaaa tcagagggca ggaactgctg aacagcaaaa
     1981 agtcagatag caccacatag cagacccgcc ataaaacgcc ctgagaagcc cgtgacgggc
     2041 ttttcttgta ttatgggtag tttccttgca tgaatccata aaaggcgcct gtagtgccat
     2101 ttacccccat tcactgccag agccgtgagc gcagcgaact gaatgtcacg aaaaagacag
     2161 cgactcaggt gcctgatggt cggagacaaa aggaatattc agcgatttgc ccgagcttgc
     2221 gagggtgcta cttaagcctt tagggtttta aggtctgttt tgtagaggag caaacagcgt
     2281 ttgcgacatc cttttgtaat actgcggaac tgactaaagt agtgagttat acacagggct
     2341 gggatctatt ctttttatct ttttttattc tttctttatt ctataaatta taaccacttg
     2401 aatataaaca aaaaaaacac acaaaggtct agcggaattt acagagggtc tagcagaatt
     2461 tacaagtttt ccagcaaagg tctagcagaa tttacagata cccacaactc aaaggaaaag
     2521 gacatgtaat tatcattgac tagcccatct caattggtat agtgattaaa atcacctaga
     2581 ccaattgaga tgtatgtctg aattagttgt tttcaaagca aatgaactag cgattagtcg
     2641 ctatgactta acggagcatg aaaccaagct aattttatgc tgtgtggcac tactcaaccc
     2701 cacgattgaa aaccctacaa ggaaagaacg gacggtatcg ttcacttata accaatacgc
     2761 tcagatgatg aacatcagta gggaaaatgc ttatggtgta ttagctaaag caaccagaga
     2821 gctgatgacg agaactgtgg aaatcaggaa tcctttggtt aaaggctttg agattttcca
     2881 gtggacaaac tatgccaagt tctcaagcga aaaattagaa ttagttttta gtgaagagat
     2941 attgccttat cttttccagt taaaaaaatt cataaaatat aatctggaac atgttaagtc
     3001 ttttgaaaac aaatactcta tgaggattta tgagtggtta ttaaaagaac taacacaaaa
     3061 gaaaactcac aaggcaaata tagagattag ccttgatgaa tttaagttca tgttaatgct
     3121 tgaaaataac taccatgagt ttaaaaggct taaccaatgg gttttgaaac caataagtaa
     3181 agatttaaac acttacagca atatgaaatt ggtggttgat aagcgaggcc gcccgactga
     3241 tacgttgatt ttccaagttg aactagatag acaaatggat ctcgtaaccg aacttgagaa
     3301 caaccagata aaaatgaatg gtgacaaaat accaacaacc attacatcag attcctacct
     3361 acgtaacgga ctaagaaaaa cactacacga tgctttaact gcaaaaattc agctcaccag
     3421 ttttgaggca aaatttttga gtgacatgca aagtaagcat gatctcaatg gttcgttctc
     3481 atggctcacg caaaaacaac gaaccacact agagaacata ctggctaaat acggaaggat
     3541 ctgaggttct tatggctctt gtatctatca gtgaagcatc aagactaaca aacaaaagta
     3601 gaacaactgt tcaccgttag atatcaaagg gaaaactgtc catatgcaca gatgaaaacg
     3661 gtgtaaaaaa gatagataca tcagagcttt tacgagtttt tggtgcattt aaagctgttc
     3721 accatgaaca gatcgacaat gtaactacta gagctaaata cattcaaata tctatccgct
     3781 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga atatgagtat
     3841 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3901 tcacccagaa acgctggtga aagtaaaaga tgccgaagat cagttgggtg cacgtgtggg
     3961 ttacatcgaa ctggacctca acagcggtaa gattcttgag agttttcgcc ccgaagaacg
     4021 tttcccaatg atgagcactt ttaaagttct gctctgtggc gcggtattat cccgtattga
     4081 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4141 ctcaccagtc acagaaaagc atcttacgga cggcatgaca gtacgcgaat tatgcagcgc
     4201 tgccataacc atgagtgata acacggcggc caacttactt ctgacaacga tcggaggacc
     4261 gaaggagctt accgcttttt tgcacaacat gggtgatcat gtaactcgcc ttgatcgttg
     4321 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4381 tatggcaaca acgttgcgca aactcttaac tggcgaactt cttactctcg cttcccggca
     4441 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4501 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt cccgcggtat
     4561 tattgcagcc ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4621 gagccaggca actatggacg aacgtaatcg ccagatcgct gagataggtg cctccctgat
     4681 taagcattgg taataatact agctccggca aaaaaacggg caaggtgtca ccaccctgcc
     4741 ctttttcttt aaaaccgaaa agattacttc gcgtttgcca cctgacgtct aagaaaagga
     4801 atattcagca atttgcccgt gccgaagaaa ggcccacccg tgaaggtgag ccagtgagtt
     4861 gattgctacg taattagtta gttagccctt agtgactctg ccacctgacg tctaagaaaa
     4921 ggaatattca gcaatttgcc cgtgccgaag aaaggcccac ccgtgaaggt gagccagtga
     4981 gttgattgct acgtaattag ttagttagcc cttagtgact c
//
